Purification of specific DNA sequences by sulfhydryl-Sepharose chromatography of mercurated polynucleotides.
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منابع مشابه
Purification of specific DNA sequences by sulfhydryl-Sepharose chromatography of mercurated polynucleotides.
Recombinant plasmid DNA has been used to purify complementary cDNA by hybridization using a modification of sulfhydryl-Sepharose chromatography described by Dale and Ward ((1975) Biochemistry 14, 2458). Plasmid DNA containing cloned mouse globin or immunoglobulin sequences was mercurated and hybridized in solution to unpurified cDNA. The resulting hybrids were passed over a sulfhydryl-Sepharose...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1978
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)34531-3